Andrew S. Greene, Ph.D.
Professor and Director, Biotechnology and Biomedical Engineering Center
Phone: (414) 456-8532 Lab website: bbc.mcw.edu
B.S. Biomedical & Electrical Engineering, Syracuse University, 1980
Ph.D. Biomedical Engineering, Johns Hopkins University, 1985
In a broad sense, our laboratory is attempting to understand fundamental principles of cardiovascular regulation and disease. Our research is highly collaborative and spans the areas of molecular and cellular biology, genetics, proteomics, technology development and microcirculatory experiments. Studies have focused on mechanisms of blood vessel growth and regression as well as the control of blood flow to tissues such as skeletal muscle and brain. We measure gene and protein expression, receptor numbers, oxygen transport, local blood flow, and microvascular density to assess the ability of the cardiovascular microcirculatory network to meet the needs of the tissue.
We perform in vivo experiments using Positron Emission Tomography, Magnetic Resonance Imaging and spectroscopy to measure changes in blood flow and metabolism in patients and animal models of disease as an aid to understanding basic mechanisms. Unique animal models produced with the aid of genomic manipulation are used in these experiments in an attempt to connect genes with cardiovascular functions.
Our lab data is analyzed with the aid of mathematical and computer models that we have developed based on morphological and experimental data. These models have proven to be extremely valuable in understanding the complex signals that cause changes in the circulation in response to normal and disease processes.
de Resende MM, Amaral SL, Moreno C, Greene AS. Congenic strains reveal the effect of the renin gene on skeletal muscleangiogenesis induced by electrical stimulation. Physiol Genomics. 2008 Mar 14;33(1):33-40.
Smith JR, Matus IR, Beard DA, Greene AS. Differential expression of cardiac mitochondrial proteins. Proteomics. 2008 Feb;8(3):446-62.
Smith JR, Matus I, Greene AS. A comparison of 2-D chromatography separations using UV and (18)O quantification of proteins in similar proteomes. J Sep Sci. 2008 Feb;31(2):314-20.
de Resende MM, Greene AS. Effect of ANG II on endothelial cell apoptosis and survival and its impact onskeletal muscle angiogenesis after electrical stimulation. Am J Physiol Heart Circ Physiol. 2008 Jun;294(6):H2814-21.
Petersen MC, Greene AS. Inhibition of angiogenesis by high salt diet is associated with impaired muscleperformance following chronic muscle stimulation. Microcirculation. 2008 Jul;15(5):405-16.
Freed JK, Shortreed MR, Kleefisch CJ, Smith LM, Greene AS. Revealing the role of phosphatidylserine in shear stress-mediated protection inendothelial cells. Endothelium. 2008 Jul-Aug;15(4):225-30.
Kriegel AJ, Greene AS. Substitution of Brown Norway chromosome 16 preserves cardiac function with aging in a salt-sensitive Dahl consomic rat. Physiol Genomics. 2008 Dec;12;36(1):35-42.
Freed JK, Smith JR, Li P, Greene AS. Isolation of signal transduction complexes using biotin and crosslinkingmethodologies. Proteomics. 2007 Jul;7(14):2371-4.
Smith JR, Olivier M, Greene AS. Relative quantification of peptide phosphorylation in a complex mixture using 18Olabeling. Physiol Genomics. 2007 Oct 22;31(2):357-63.
Hicks WA, Halligan BD, Slyper RY, Twigger SN, Greene AS, Olivier M. Simultaneous Quantification and Identification Using (18)O Labeling with an Ion Trap Mass Spectrometer and the Analysis Software Application "ZoomQuant." J Am Soc Mass Spectrom. 2005 Jun;16(6):916-25.
Halligan BD, Slyper RY, Twigger SN, Hicks W, Olivier M, Greene AS. ZoomQuant: an application for the quantitation of stable isotope labeled peptides. J Am Soc Mass Spectrom. 2005 Mar;16(3):302-6.
Drenjancevic-Peric I, Greene AS, Kunert MP, Lombard JH. Arteriolar responses to vasodilator stimuli and elevated P(O2) in renin congenic and Dahl salt-sensitive rats. Microcirculation. 2004 Dec;11(8):669-77.
Liang M, Cowley AW, Greene AS. High throughput gene expression profiling: a molecular approach to integrative physiology. J Physiol. 2004 Jan 1;554(Pt 1):22-30.
Santos CF, Caprio MA, Oliveira EB, Salgado MC, Schippers DN, Munzenmaier DH, Greene AS. Functional role, cellular source, and tissue distribution of rat elastase-2, an angiotensin II-forming enzyme. Am J Physiol Heart Circ Physiol. 2003 Aug;285(2):H775-83.
Amaral SL, Maier KG, Schippers DN, Roman RJ, Greene AS. CYP4A metabolites of arachidonic acid and VEGF are mediators of skeletal muscle angiogenesis. Am J Physiol Heart Circ Physiol. 2003 May;284(5):H1528-35.